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immortalized mouse podocyte and tubular cell lines  (Charles River Laboratories)

 
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    Structured Review

    Charles River Laboratories immortalized mouse podocyte and tubular cell lines
    ( A–D <t>)</t> <t>Immortalized</t> <t>podocytes</t> and tubular cells of wild-type (WT) and AS mice treated with empagliflozin (Empa) or vehicle for 48 hr. ( A,C ) Bar graph analysis showing cytotoxicity normalized to viability, then compared to WT (n=3). ( B,D ) Bar graph analysis showing apoptosis normalized to viability, then compared to WT (n=3). ( E,F ) LD accumulation in tubular cells ( E ) and podocytes ( F ) was measured by Nile red staining. Bar graph analysis showing the quantification of the number of LDs per cell (n=3). ( G ) Correlation analyses between the LD accumulation and apoptosis in podocytes (n=12). ( H ) Representative images of Nile red staining demonstrate increased LD numbers (Nile red: green) in AS podocytes (Cell mask blue: blue; DAPI: red) compared to WT podocytes, which is reduced by Empa treatment. ( A–F ), Two-tailed Student’s t-test. ( G ), Pearson’s correlation coefficient. *p<0.5, **p<0.01.
    Immortalized Mouse Podocyte And Tubular Cell Lines, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/immortalized+mouse+podocyte+and+tubular+cell+lines/conditionally+immortalized+podocyte+cell+line/pmc10185338-241-3-25
    Average 90 stars, based on 1 article reviews
    immortalized mouse podocyte and tubular cell lines - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Empagliflozin reduces podocyte lipotoxicity in experimental Alport syndrome"

    Article Title: Empagliflozin reduces podocyte lipotoxicity in experimental Alport syndrome

    Journal: eLife

    doi: 10.7554/eLife.83353

    ( A–D ) Immortalized podocytes and tubular cells of wild-type (WT) and AS mice treated with empagliflozin (Empa) or vehicle for 48 hr. ( A,C ) Bar graph analysis showing cytotoxicity normalized to viability, then compared to WT (n=3). ( B,D ) Bar graph analysis showing apoptosis normalized to viability, then compared to WT (n=3). ( E,F ) LD accumulation in tubular cells ( E ) and podocytes ( F ) was measured by Nile red staining. Bar graph analysis showing the quantification of the number of LDs per cell (n=3). ( G ) Correlation analyses between the LD accumulation and apoptosis in podocytes (n=12). ( H ) Representative images of Nile red staining demonstrate increased LD numbers (Nile red: green) in AS podocytes (Cell mask blue: blue; DAPI: red) compared to WT podocytes, which is reduced by Empa treatment. ( A–F ), Two-tailed Student’s t-test. ( G ), Pearson’s correlation coefficient. *p<0.5, **p<0.01.
    Figure Legend Snippet: ( A–D ) Immortalized podocytes and tubular cells of wild-type (WT) and AS mice treated with empagliflozin (Empa) or vehicle for 48 hr. ( A,C ) Bar graph analysis showing cytotoxicity normalized to viability, then compared to WT (n=3). ( B,D ) Bar graph analysis showing apoptosis normalized to viability, then compared to WT (n=3). ( E,F ) LD accumulation in tubular cells ( E ) and podocytes ( F ) was measured by Nile red staining. Bar graph analysis showing the quantification of the number of LDs per cell (n=3). ( G ) Correlation analyses between the LD accumulation and apoptosis in podocytes (n=12). ( H ) Representative images of Nile red staining demonstrate increased LD numbers (Nile red: green) in AS podocytes (Cell mask blue: blue; DAPI: red) compared to WT podocytes, which is reduced by Empa treatment. ( A–F ), Two-tailed Student’s t-test. ( G ), Pearson’s correlation coefficient. *p<0.5, **p<0.01.

    Techniques Used: Staining, Two Tailed Test

    Related Articles

    Crocin Bleaching Assay:

    Article Title: Empagliflozin reduces podocyte lipotoxicity in experimental Alport syndrome
    Article Snippet: Sections were examined under a light microscope (Olympus BX41, Tokyo, Japan). .. To establish immortalized mouse podocyte and tubular cell lines, Col4a3 +/- mice were bred with the immorto-mice carrying a temperature-sensitive T-antigen transgene (SV40 + ) (Charles River, Wilmington, MA, CBA/CaxC57BL/10-H-2Kb-tsA58) ( ) to generate double heterozygous littermates, which were then crossed to generate SV40 + ; Col4a3 -/- (immorto-AS) and SV40 + ; Col4a3 +/+ (immorto-WT) ( ; ). ..



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    immortalized mouse podocyte and tubular cell lines  (Charles River Laboratories)
    90
    Charles River Laboratories immortalized mouse podocyte and tubular cell lines
    ( A–D <t>)</t> <t>Immortalized</t> <t>podocytes</t> and tubular cells of wild-type (WT) and AS mice treated with empagliflozin (Empa) or vehicle for 48 hr. ( A,C ) Bar graph analysis showing cytotoxicity normalized to viability, then compared to WT (n=3). ( B,D ) Bar graph analysis showing apoptosis normalized to viability, then compared to WT (n=3). ( E,F ) LD accumulation in tubular cells ( E ) and podocytes ( F ) was measured by Nile red staining. Bar graph analysis showing the quantification of the number of LDs per cell (n=3). ( G ) Correlation analyses between the LD accumulation and apoptosis in podocytes (n=12). ( H ) Representative images of Nile red staining demonstrate increased LD numbers (Nile red: green) in AS podocytes (Cell mask blue: blue; DAPI: red) compared to WT podocytes, which is reduced by Empa treatment. ( A–F ), Two-tailed Student’s t-test. ( G ), Pearson’s correlation coefficient. *p<0.5, **p<0.01.
    Immortalized Mouse Podocyte And Tubular Cell Lines, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/immortalized+mouse+podocyte+and+tubular+cell+lines/conditionally+immortalized+podocyte+cell+line/pmc10185338-241-3-25
    Average 90 stars, based on 1 article reviews
    immortalized mouse podocyte and tubular cell lines - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A–D ) Immortalized podocytes and tubular cells of wild-type (WT) and AS mice treated with empagliflozin (Empa) or vehicle for 48 hr. ( A,C ) Bar graph analysis showing cytotoxicity normalized to viability, then compared to WT (n=3). ( B,D ) Bar graph analysis showing apoptosis normalized to viability, then compared to WT (n=3). ( E,F ) LD accumulation in tubular cells ( E ) and podocytes ( F ) was measured by Nile red staining. Bar graph analysis showing the quantification of the number of LDs per cell (n=3). ( G ) Correlation analyses between the LD accumulation and apoptosis in podocytes (n=12). ( H ) Representative images of Nile red staining demonstrate increased LD numbers (Nile red: green) in AS podocytes (Cell mask blue: blue; DAPI: red) compared to WT podocytes, which is reduced by Empa treatment. ( A–F ), Two-tailed Student’s t-test. ( G ), Pearson’s correlation coefficient. *p<0.5, **p<0.01.

    Journal: eLife

    Article Title: Empagliflozin reduces podocyte lipotoxicity in experimental Alport syndrome

    doi: 10.7554/eLife.83353

    Figure Lengend Snippet: ( A–D ) Immortalized podocytes and tubular cells of wild-type (WT) and AS mice treated with empagliflozin (Empa) or vehicle for 48 hr. ( A,C ) Bar graph analysis showing cytotoxicity normalized to viability, then compared to WT (n=3). ( B,D ) Bar graph analysis showing apoptosis normalized to viability, then compared to WT (n=3). ( E,F ) LD accumulation in tubular cells ( E ) and podocytes ( F ) was measured by Nile red staining. Bar graph analysis showing the quantification of the number of LDs per cell (n=3). ( G ) Correlation analyses between the LD accumulation and apoptosis in podocytes (n=12). ( H ) Representative images of Nile red staining demonstrate increased LD numbers (Nile red: green) in AS podocytes (Cell mask blue: blue; DAPI: red) compared to WT podocytes, which is reduced by Empa treatment. ( A–F ), Two-tailed Student’s t-test. ( G ), Pearson’s correlation coefficient. *p<0.5, **p<0.01.

    Article Snippet: To establish immortalized mouse podocyte and tubular cell lines, Col4a3 +/- mice were bred with the immorto-mice carrying a temperature-sensitive T-antigen transgene (SV40 + ) (Charles River, Wilmington, MA, CBA/CaxC57BL/10-H-2Kb-tsA58) ( ) to generate double heterozygous littermates, which were then crossed to generate SV40 + ; Col4a3 -/- (immorto-AS) and SV40 + ; Col4a3 +/+ (immorto-WT) ( ; ).

    Techniques: Staining, Two Tailed Test